Is there any way to determine which amino acids of IgG will the biotin bind to during biotinylation?

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Dominique Liger

Hi there,

Biotinylation may occur on different chemical groups (primary amine, thiol, carboxylic acid, aldehyde) depending on the protocol and reagent used. This handbook should be helpful:

https://tools.thermofisher.com/content/sfs/brochures/1601675-Avidin-Biotin-Handbook.pdf

Aditya Kurdekar

Thanks a lot!!!!

Md Babu Mia

Hello Mr Aditya Kurdekar ,

Biotin can bind with dfferent chemical groups, but binding with IgG is still on queue. Normally, biotin bind with primary amines in cell. Are you doing this binding experiment in vitro ?

Best regards,

Babu