I have immobilized a cellulase enzyme on a hydrogel support. As we know, the structure of the enzyme and availability of its active site are effective factors in its activity, on the other hand, the enzyme immobilization changes its three-dimensional structure. I would like to examine the effect of this immobilization on the enzyme structure.
Dear Afsaneh,
I remember reading an article, which suggested the use of SIRMS to study the structure of immobilized enzymes.
Although there is no way to check the 3-D structure of an immobilized enzyme directly, in most cases it can be assumed that it is not significantly modified by immobilization. This is borne out by the fact that immobilized enzymes retain activity, even if their kinetic parameters may be modified by immobilization
Dear Navnit and Pierre,
Thank you very much. I am very grateful of you.
Hydrogel immobilization of enzyme may not affect the 3-D structure but I think immobilization of enzymes like cellulases, which have insoluble/ cellulosic polysaccharide substrate will reduce the enzyme activity due to hindrance in substrate passing though the gel barrier.
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