It's required to detect the concentration of ammonium sulfate in a solution.
Can you titrate with some precipitating agents such as barium chloride?
You can use the Nessler's reagent.
See the following ResearchGate question for some aspects
https://www.researchgate.net/post/Should_there_be_resulting_precipitate_formation_in_a_Nesslers_reagent
There are methods for determination of total nitrogen that are accurate but a little cumbersome to use. In principle you make the solution alkaline, distill the ammonium off and titrate it with HCl.
The ammonium content can be liberated in the form of ammonia gas from basic solution and titrate with standard HCl as suggested by Jukka Salminen. The sulfate content could be determined by precipitation titration with standard barium chloride solution. Both methods could work but should also be carefully checked for interferences by any other compounds/chemicals present in the solution.
Gives us the exact figure: wich volume of serum was used, which saturation (%) ? Did you taken the precipitate ? In which volume this was dissolved ?
400 ml of serum precipitated with 260 ml (fully saturated H2O with ammonium sulfate), then centrifuge the mixture and save the pellets (1 tenth original volume), I need to figure the ammonium sulfate concentration in these pellets, thank you very much
Wash your precipitate a couple of times with freshly deionized water. Then measure the ammonium from the pooled supernatant. A commercial ammonia meter (e.g. PocketChem BA Blood) would be easiest to use.
You can have an estimate of the ammonium sulfate (AS) concentration. The conditions you used mean a final 40% saturation that is aproximately 0,23 g/mL of AS. This will be the maximum concentration in the pellet after centrifugation.
Lets take an example, assuming that the volume of the pellet after centrifugation was 10 mL (you can afterwards ajust to your real volume !).
Assume that the maximum AS concentration in the pellet is as in the solution, thus 0,23g/mL meaning 2,3 g total AS.
Then if you dissolve your pellet in a final volume of 50 mL, the final concentration in the solution is about 0,046g/mL.
I hope that this will help !
- Badges
- Science topic
- Similar topics
- Chemistry