Seeds of Viola odorata germinate hardly and we have low emergency in the field. we can propagate it via stolon.Can anyone say a good experience to increase seed germination percentage?
Viola would need stratification treatment for proper germination. Have a look at the following website for details of the procedure. It will also be good to keep the seeds in darkness during imnbibation and germination - it might increase germination percentage. If it does not work then gibberelic acid treatment or KNO3 treatment may be a good option.
GA-3 will in most cases eliminate the need for stratification. The exact concentration required will vary between species but I have found a 24 hour soak in 500-1000 ppm aqueous GA-3 solution to be fairly effective and have not yet found it to produce excessive etiolation or other undesirable effects.
One simple thing that you can use is put the seeds in cotton embedded in water, after this, keep in storage cold (refrigerator - avoid freezing) during 4 to 6 weeks. Look this during all days until germination. After germination just change for a potted with soil.
What is important is to understand the type of dormancy is Viola odorata that's the bases we can use to determine whats the best method to break such type of dormancy.
You can use Agrostemin. Either mix seeds with powder or spray solution on seeds before planting or dip seeds in solution. You will have a considerable increase in germination.
Another effect will be the increase in most if not all phenols. If plants will be used for medicinal purposes, caution with roots, will contain higher amounts of alkaloids(more potent)
Dear Saeid have you done any experiment in pretreatment for breaking the seed dormancy?what was the result? Is it reproduceable or not? have you got two cleistogamouse and chasmogamouse seed image? How long these seeds keep their viability?