How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to correctly choose the number of solution intervals when preforming harmonic analysis in ANSYS?

Good day, I design the rail-wheel geometry and the harmonic frequency ranges between 20-10kHz with 500Hz solution intervals and the results makes sense. Most of the time on the related literatures...

19 February 2021 3,190 3 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

01 February 2021 3,735 3 View

What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

How to compute the phase from a fringe pattern using Hilbert Huang Transform in MATLAB?

I have computed line Wise IMF from the fringe data using empirical mode decomposition (EMD). For each line Segment I have more than five IMF sets but i am not able to understand that how to...

10 January 2021 5,036 1 View

Which statistical test is best to run on a heavily right-skewed dependent variable?

My dependent variable when graphed is heavily right-skewed. All attempts to transform it has failed (Log, Log10, Sqrt, ..). The most ideal test I would like to run is a multiple linear...

07 January 2021 2,705 4 View

How can I estimate and plot Logarithmic Error Bars (in Excel, preferably)?

Dear Dr.colleagues, I am currently plotting the dynamics of an infection in Excel. For each sampling day, I plotted the mean infection load of the population, using the standard error for the...

07 January 2021 2,069 3 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View