Is there any tool (freely available online) to identify protein binding sites in a protein-protein interaction?

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Is Bmax value greater than 1 correct in a radioligand binding assay?

I use sigma plot software, its a radioligand binding assay for determination of Kd values for the Aptamer-protein binding. I use one site saturation.

02 March 2018 5,570 3 View

How to remove the unincorporated labeled nucleotides from the sample eDNA ?

I have been using illustra microspin G50 columns for getting rid of the unincorporated labeled nucleotides from my sample eDNA. But unfortunately this does not seem to work. I still end up with...

01 February 2017 6,386 10 View

Can furin be expressed effectively in insect (SF9) cell line? Trying to make a continuos cell line expressing furin. Suggestions needed?

Furin is expressed as a proprotein, so i had included a myc tag after its cleavage site on the n terminal position. have confirmed the tag by sequencing. Aptly, i need to see the band on a western...

01 January 1970 7,798 2 View

Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Is it true that science is leaving the era of mathematics and entering the era of matrix mechanics?

We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...

31 July 2024 3,900 2 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

A Question about Phd thesis?

Hello everyone What is your opinion about the introduction of an expert decision support system in which the rules are extracted from existing data without human intervention, instead of being...

31 July 2024 5,785 4 View