Chandan Narayan

4 Questions 10 Answers 0 Followers

Questions related from Chandan Narayan

Is Bmax value greater than 1 correct in a radioligand binding assay?

I use sigma plot software, its a radioligand binding assay for determination of Kd values for the Aptamer-protein binding. I use one site saturation.

03 March 2018 5,557 3 View

How to remove the unincorporated labeled nucleotides from the sample eDNA ?

I have been using illustra microspin G50 columns for getting rid of the unincorporated labeled nucleotides from my sample eDNA. But unfortunately this does not seem to work. I still end up with...

02 February 2017 6,373 10 View

Is there any tool (freely available online) to identify protein binding sites in a protein-protein interaction?

For example, if we have two proteins, is there any way to detect the binding sites (portion of the molecules) with the highest affinity in silico, before actually performing the experiments?

03 March 2014 9,877 3 View

Can furin be expressed effectively in insect (SF9) cell line? Trying to make a continuos cell line expressing furin. Suggestions needed?

Furin is expressed as a proprotein, so i had included a myc tag after its cleavage site on the n terminal position. have confirmed the tag by sequencing. Aptly, i need to see the band on a western...

01 January 1970 7,780 2 View