I'm going to amplify mature miRs, but I have to design primers for RT-PCR myself. so, Is there any suitable software or protocol for stem-loop primer design?
Ali Hamoudi
Take a look at this:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1292995/
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Fatemeh Mirzadeh
thank you Ali, i've also reviwed a protocol by Varkonyi-Gasic et al., but i need a software to evaluate my proposed primers
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