Hello,

there are several methods for the extraction of chitin: chemical (deproteinisation by use use alkakine treatment), biological (of of protease) or gravimetric methods (sufficient weight of samples). You must adapte the methods according to your sample (weight) and the further analysis planed (objectives of the study, use of radiolabelled precursors of chitin...). See attached two publications dealing with the extraction: the first one with inscet mùodels and the secund one in crustacean species (review of the different methods) with data on several organisms such as insects for comparison. Finally, you find for more details three publication made on chitin (gravimetric method and study of chitin synthesis using radiollabelled precursors of chitin). Do not hesitate tio contact me for more infromation.

Good luck !

Prof. Noureddine Soltani

Sir thanks for such a detailed answer, but i asked about chitin extraction from compound eye of any insect. my question was that is there any study upto now in the literature about this subject?

Thanks for your intrest

Regards

Muhammad Mujtaba

Hello Muhammed,

There are not information about chitin from compound eye in the the literatur in my kwnowledge. .However, the methods can be adapted to your sample.

Best regards Pr. N. Soltani

ok thank you sir for your intrest.

Regards 

Muhammad MUjtaba