Is there any spectrophotometric assay for determining the endotoxin / LPS degrading capability of a compound/ extract?

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What could be the career options after a Ph.D. in Herbal Drug Discovery?

Respected Researchers and my fellow colleagues, I am on the verge of completing my Ph.D. in the field of Microbiology with specific focus on 'Development of Herbal based Bio-protective agents...

11 December 2015 9,165 2 View

Herbal extracts related manuscripts dont get published as compared to synthetic drugs, even when 70% of synthetic drugs have plants as precursor

Why the future of herbal in the world is still very dark, despite of the fact that only holistic interventions of herbals can mitigate problems like antibiotic resistance?

10 November 2015 3,572 5 View

What is the fundamental reason associated with toxicity of purified phyto-constituents?

When we go down the lane of herbal drug extraction, we undergo the process of bioactivity guided fractionation, which further paves the way for purification of the desired herbal phytoconstituent....

10 November 2015 4,075 13 View

What could be the underlying reason behind antagonistic behaviour of certain herbals when taken along with chemotherapeutic synthetic drugs?

Certain natural plant products show contraindications when taken along with an ongoing synthetic drug regime. What could be the most probable cause of such antagonistic behaviour shown by herbals?

10 November 2015 7,359 5 View

Plight of Ph.D. Students in India

I would like to ask you all that despite of Ph.D. being the highest degree in India, why is it that the Ph.Ds are still facing the challenges of unemployment.....They are still applying for low...

01 January 1970 5,622 7 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View