Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

How to estimate the extent to which the FE domain should be meshed with explicit elements in Explicit-Implicit Time integration procedure?

I am developing a MATLAB code for shock problem and want to divide the FE domain into explicit and implicit elements (region of domain at vicinity to shock is meshed into explicit elements and...

15 January 2021 1,968 2 View

How to adjust a covariate in meta-analysis?

Suppose I am meta-analyzing proportions using their logit transformations. We did a meta-regression of the covariates age, gender, and disease severity score in studies and get respective beta...

11 January 2021 9,409 4 View

Is there any specific software available for graphical presentation of signaling pathways/cycle in cells?

I'm planning to write a Review article, to make it more worthy and easy to understand for the targeted audience, I'm thinking to add some signalling pathways, and unfortunately, I have no idea how...

30 December 2020 6,395 2 View

Is it possible that first waiting time decreases and then after a certain point waiting time start increasing?

The sevice rate is fixed and the arrival rate is changing. As I am plotting the graph of arrival rate with respect to waiting time in queue.

22 December 2020 2,455 1 View

What could be the possible reason for lack of Mtb protein expression cloned in pET28a even after induction ?

12 December 2020 2,584 8 View

Can anyone help me to find research paper of "Banks, W. H. H"?

Dear researchers, I need this research paper "Similarity solutions of the boundary-layer equations for a stretching wall by Banks, W. H. H (Bristol, University, Bristol, England)." If anyone...

07 December 2020 2,157 1 View

Why the IT services companies (based in India) are mainly patenting only at the USPTO?

Please suggest some papers/reports on the changes in the policy with respect to patenting of computer software in India. Does it have any implications as to why the IT services companies (mostly...

12 November 2020 8,351 1 View

How can I simulate noise of CMOS Image Sensor by sentaurus TCAD?

Can anyone tell me how to simulate noise of CMOS image sensors, such as thermal noise and flicker noise with sentaurus TCAD?

09 November 2020 9,616 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

How to resolve Gaussian error termination " Error termination via Lnk1e in F:\G16W\l103.exe" for TS(QST3)?

When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...

21 February 2021 3,198 2 View

What effect does the large temperature difference between the two primers have on PCR, including real time PCR?

What effect does the large temperature difference between the two primers have on PCR, including real time PCR? If I did not get a result from real time PCR, is it possible that my problems are...

21 February 2021 4,094 7 View