Is there any site for blasting Bisulfite sequencing PCR (BSP) primers?

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How to determine the false positive sequencing result?

Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After...

17 January 2021 3,263 11 View

Why did the authors design a sociodemographic questionnaire?

This is a question from a journal reviewer who asked my manuscript. Can someone please guide me on how to prepare the reviewer's answer? Thanks. here is reviewer question. why did the authors...

16 November 2020 735 2 View

How can I fix the capacity curve of two different structure?

I have two frames for nonlinear analysis, one of them is CBFs and one of them is BRBFs ( the generals are the same, only lateral elements are different). When I drew the capacity curve of my...

11 November 2020 10,034 3 View

Why the initial pressure in a fluid cavity simulation in Abaqus fall to zero just at the starting increments?

I am using fluid cavity capability for simulation of a water-filled container with Abaqus-Static-Standard. The container has an initial pressure and is been surrounded by another solid layer with...

21 March 2020 7,153 3 View

How can I save normal directions of solid elements in Abaqus?

Dear all, I need to have normal directions of solid elements in my 3D simulation with Abaqus. I know that in Visualization Module, Options tab> common or superimposed, there is the possibility...

28 February 2020 2,703 3 View

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I'm trying to collect milk from our knockout mice (at the end of the lactation stage) to isolate milk exosomes. But I have a hard time with milk collection from mice. I have used either pump or...

22 January 2020 4,527 1 View

How can we increse the osmotic pressure of a solution while maintaining the electrical conductivity of the solution?

in other words what kind of salts increase the osmotic pressure with the same salt content. for example 1g of NaCl or 1 gr of Na2SO4 or KCl or ....

25 January 2019 2,610 3 View

What is curtain gas in LC-MS technique?

is collision gas is the same with curtain gas in LC-MS technique? and as a whole what is curtain gas in LC-MS?

22 January 2019 2,823 5 View

Dental decision support systems by Image Processing and Data Mining?

Intelligent Therapist Designer for Dentistry with Data mining and image processing

18 January 2019 9,288 3 View

Surface treatment and finishing for BiFeO3?

Hello everyone, I want to do surface treatment for my sol-gel synthesized BiFeO3. For this purpose if anyone have a kind suggestions regarding methodology that how to do and which way is better...

26 December 2018 7,295 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View