Histology is a powerful technique to see the structural changes in the cellular and tissue level. But the whole process at lease 48 hrs to be completed. Is there any short-cut so that we can minimize the time as well as maintain good quality slides?
Dear Muhammad
i did both shorten and long the time for histology.
in my experience when you do shorten during histology process and actually in Dehydration stage, tissue wont be Dehydrated as well in longer time. your tissue should be in various alcohol about 7-10 hrs.
next stage and in Paraffinization you should do it shorten again. about 10-12 hrs.
i did histology of gonad, liver and intestine of various fish like zebrafish.
if you need help you can asked me personally.
best regards.
Dear Dr. Mahdi, thank you for appreciable answer. By the way can you share more information on frozen sections?
Dear Mr. Hamed, thank you for much appreciated answer as you are scientist in this field, many more things we will share-
Your question look ambiguous because histology slide preparation is diverse. Is it for Plant specimen or animal specimen if it is for any which part of the organism? Because preparation of specimen for histological studies varies.
Yea, I didn't mention which specimen, actually fish tissue, such as liver, kidney, gut, gills etc.
Try needle "biopsy" sizes of tissue, they can go through an enclosed tissue processor such as teh Leica ASP300S within 2 hours including time for fixation. Or snap frozen & cryostat sections. Or you can sometimes "blot" a fresh unfixed tissue onto a slide leaving a finger print of that tissue. I would back all of these up however with traditional FFPE tissues..
The only short -cut is if your specimen can be cut on sledge or freehand section which need no embeddement.
Dear Dr. Matthew, thank you so much for your highly appreciated answer. After all you are the expert in this field.
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue