In order to simulate a DWC in steady state, how to put the unit operations in the flowsheet of any process simulator?

14 January 2021 3,938 6 View

Which is the best bibliography to search for experimental data of process engineering?

16 December 2020 7,894 2 View

How to set Decouplers in control systems?

09 December 2020 1,409 5 View

What are the antimicrobial categories and agents that are currently considered to define a MDR, XDR and PDR Salmonella?

What antimicrobial categories and agents are currently considered to define non-typhoid Salmonella as multidrug resistant (MDR), extremely / extensively drug resistant (XDR) or pandrug resistant...

19 November 2020 755 3 View

How to built a simulation of a chemical process?

12 September 2020 7,685 10 View

Mask for outdoor exercise during covid pandemics?

Hi !! I've seen a lot of arguments in favour and against the use of a mask while running outdoors. I think it's just that some people are not comfortable enoughwearing one, but maybe there is some...

08 July 2020 4,927 2 View

How to implement Ergun equation in COMSOL?

06 May 2020 5,701 1 View

No title

I've been trying to obtain a 2D-PAGE from a protein extract, but I haven't obtained an adequate result. So far, I've obtained gels with poor proteic content or with an artefact in the lower...

08 March 2020 4,339 3 View

In a control structure, how could I put four controllers (either PI or PID) for a distillation column?

I put four controllers in a distillation column at the beginning they all work pretty well, however, after setting another controller, the control was getting worse (increasing IAE values).

16 February 2020 2,865 3 View

Why the JCR journal -Annali di Botanica- is not included the list of journals of Research Gate?

The journal "Annali di Botanica" that appears in the JCR journal list (Plant Sciences category; Q3; position 170/28 in 2018) is not included in the Research Gate journals.

29 January 2020 6,930 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View