Hi Rajdeep
its only dependent on optimum growth temperature of the organism (bacteria), for example if the optimum growth temperature of X bacteria is 30 C and been incubated at this temperature (30 C) or very close (more or less) then it will degrade or use magnesium and calcium, some bacteria has a wider range that could be 10 C (or even greater) less or more than the optimum temperature and are able to metabolise these minerals.
Hope this help
Theoretically yes. Magnesium or calcium ions can modulate the thermostability of proteins and nucleic acids.
In theory yes, but the effect on the bacteria depends on the bacterial strains/genera you are using. As previously stated, magnesium or calcium ions can modulate the thermostability of proteins and nucleic acids.Extreme temperatures (very low or really high also affect calcium/magnesium solubilisation), so you have to keep in mind this effect too.
Finally, it is also very important to chose proper calcium/magnesium salts to do theses experiments, magnesium sulphate for instace retains water, so it is not the best salt to do biochemical characterisations or to prepare culture media/buffers
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