PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

How many indicators do I need to represent a latent construct?

Hi folks! We did a large survey and planned to use 3 indicators to represent a latent construct (Sense of Place/SoP) in a latent regression model (structural equation modeling). However, one of...

15 February 2021 7,705 3 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

What is the structure of a photon that can describe the interference experiments that reject the wave nature of light?

De Broglie's double-solution postulated a causal model of Quantum Mechanics. Knowledge of the photon structure was needed to model the particle part of the cause of the Double Slit interference...

11 February 2021 6,869 5 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View

Could you please interpret the data and SAXS patterns of lipid nanoparticles for us?

Dear Prof. Weiss, Recently, we prepared lipid nanoparticles (composed of phophatidylserine and aluminum ions), and we characterized the particles using SAXS. However, we are unable to interpret...

15 December 2020 4,292 3 View

Why does surface roughness enhance (super)hydrophobicity?

Hello, according to Wenzel's model, an increase in surface roughness will also increase the contact angle, but what is the thermodynamic reason for it? Considering the lotus effect, does a...

22 October 2020 9,004 8 View

Is it possible that the hydrophilicity of a scaffold decreases by increasing the concentration of graphene oxide?

The water contact angle results exhibited increased hydrophilicity by increasing the graphene oxide(0 to 0.5%w/v). Still, it occurred oppositely at the concentration of 0.75%w/v even with...

19 October 2020 553 3 View

Why is the q-value in SAXS data 2pi/d and not 1/d?

I am usually working with electron microscopy images of macromolecules, and we use reciprocal distance units [1/Angstrom] for units in Fourier Space. A repeating signal every e.g. 10 Angstrom...

27 September 2020 5,008 1 View

Asked a question in contact angle ?

03 August 2020 1,703 11 View

Do you have a specific question in glaucoma that may need a systematic review and/or a meta-analysis?

I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this...

21 May 2020 6,692 1 View

Why is in the SAXS of some hexagonal phase polymer that the third peak (200) is higher than the second peak (110)?

I have an ionic polymer that presents a hexagonal phase. The second peak (110) intensity is lower than the third peak (200), unlike usual hexagonal patterns. I found that some literature did...

26 April 2020 9,460 4 View