Was Ilya Prigogineusing extending concepts referring to change of state in dynamical systems to changes in structure such as self-orgasnization?

Why Ilya Prigogine talks about self-organization (implying structural change) in dissipative systems using the concepts of bifurcation and attactors (referring to state changes)? I could never...

15 August 2020 5,649 12 View

How do you assess children online?

The actual health crisis has changed our lives and also affected the research projects of many. I was wondering if there was a way of assessing preschool children online. I know the best way is...

26 March 2020 8,699 7 View

Has anyone tried performing a transient transfection on a cell line that has been stably infected with a lentivirus?

Hello, I was having difficulty in infecting a cell with a gene of interest. Say, for instance, I was able to introduce a CMV on the cell, but I was not successful in introducing my target gene. I...

25 September 2019 2,412 3 View

How to arrange a data set for 2 Way ANOVA?

Hello! I'm trying to run a 2 Way ANOVA analysis on a data set from an animal model. My rats were exposed either to one of two treatments during pregnancy and the offspring was later exposed to...

21 March 2019 2,167 1 View

How do you understand the relationship between problem solving and the development of higher order thinking skills (HOTS) in mathematics teaching?

I am looking for convergences between working with problem solving and the development of HOTS.

28 November 2018 5,549 3 View

Did anybody perform a succesful mode-superposition transient dynamic analisys under enforced base acceleration (MODCONT,,ON) using ANSYS Mech APDL?

I'm trying to get nodal accelerations on a simply supported beam subjected to a white noise signal through its supports. I'm following instructions given in the Ansys Manual but I can't get any...

09 October 2018 3,344 2 View

Is it allowed to transpose the allowed limits in human of a toxic to an animal model?

I work with a rat model exposed to Cd, however their blood levels do not exceed the max mentioned in the WHO. In which case, can I apply this parameter? Taking into account that it is a metal dose...

13 September 2018 2,075 1 View

What are the main methods to determine uranium, thorium and their daughters quantities in an organic sample?

How I could determine uranium quantitiy in oil?

15 June 2018 2,149 16 View

How to achieve even western blot bands?

So I have been working on this Western Blot technique for my laboratory rotation and I still get uneven bands. In my last experiment the samples are arranged in the next way: 1 - 5 and once again...

23 February 2018 6,222 4 View

Plasmid options to express a recombinant protein in clinical Providencia rettgeri strain?

25 January 2018 8,863 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View