You can add calcium and then spin at 20-45,000 rpm. I forgot the concentration of calcium. I would try to google the method.

What's your further experiment for these proteins? If you only need them to do activity assay, you can try to solubilize the supernatant with Triton/other detergent after 16000rpm. And then if enrichment is needed, you can concentrate the sample and run one cycle of gel filtration or ion exchange column.

Thanks for the reply Lin . I need it for just activity activity assay at the moment and also furthur analyse with GCMS. Il try that and would see how it goes