How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

What are the mechanism of actions of Ag and Cu nanoparticles on the bacteria and fungi?

We are assessing the activity of nanoparticle incorporated fabrics against bacteria and fungi.

24 November 2020 8,686 6 View

How to estimate nitrogen content (%) in plant using pelican equipment's KELPLUS ELITE-EX VA semi automatic kjeldahl distillation unit? ?

I am using KELPLUS ELITE-EX VA semi-automatic Kjeldahl distillation unit for nitrogen estimation. The salesman told me that unlike the older version; this version does not require making up the...

01 October 2020 844 3 View

Why Magnesium Sulfate is used in Loop-Mediated Isothermal Amplification(LAMP) reaction instead of MgCl2?

We have designed an in house LAMP assay for the detection of a pathogen. We have used ThermoPol buffer, betaine, MgSO4, Bst polymerase and dNTPs in the reaction. I wanted to know why MgSO4 is used...

22 September 2020 6,916 5 View

Cloning in TRV2 vector: tips?

So, I have been cloning a lot of genes over the years in various plant transformation and overexpression vectors and even though its a struggle always to generate positive clones, I usually am...

26 August 2020 8,364 9 View

Is it possible to hire a portable photosynthesis system in Northern India?

I have an experiment in which I want to estimate the photosynthetic activity and carbon dioxide flux of wheat plant. However, in my institute does not have potable photosynthesis equipment. Is...

11 August 2020 9,701 2 View

Is it possible to publish research paper on plant nutrition having only single year of research work in a Thompson and Reuters IF Journal?

As in Masters of Science in Agronomy in many places have only one year of research work, is it possible to convert it to a paper and publish it in an impact factored journal especially when the...

04 August 2020 4,252 6 View

Has anyone used CABS flex 2.0 for molecular dynamics simulation of docked protein-protein structures?

Hi all, I need an info on the CABS flex and how it works for protein protein interaction simulations. I have docked structures obtained using cluspro and would like to subject them to MD. Please...

24 April 2020 1,509 1 View

What is the difference between Bubble type and Vapor type Precursor?

I am working with La(PrCp)3 Precursor in my Lab. I am not getting enough thickness after oxide layer deposition with ALD. To solve this issue I want to know about the precursor's chemical...

16 February 2020 5,640 3 View

How to register an indigenous variety in India?

The farmers are cultivating an unregistered land-race for a long period; if I wish to register this variety, what are the legal procedure and how to register it.

14 February 2020 525 3 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View