Hello

some efforts were made previously base on strain complementation but were unsuccessfull.

https://www.ncbi.nlm.nih.gov/pubmed/10531227

This challenge will be very difficult since there are 53 insertions and 21 deletions in H37Ra genome compared to H37Rv one (affecting more repetitives element Like IS insertion seq and PE/PPE  family), Of note there is more than 70 SNVs soecific to H37Ra affecting more than 30 genes.

See this pub

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0002375

Dear Hafid,

                   Thank you for your answer.