Dear Thiruventhan Karunakaran,

The following paper entitled " Phytochemical, Antimicrobial and Antiprotozoal Evaluation of Garcinia Mangostana Pericarp and α-Mangostin, Its Major Xanthone Derivative " and published in Molecules 2013, 18, 10599-10608; doi:10.3390/molecules180910599 contains some important data on the detection of xanthones.

For more information on xanthones you can visit my RG profile which contains full text of some papers published on this topic.

Phytochemical, Antimicrobial and Antiprotozoal Evaluation of

Garcinia Mangostana Pericarp and α-Mangostin, Its Major

Xanthone Derivative

Abstract: Five xanthone derivatives and one flavanol were isolated from the

dichloromethane extract of Garcinia mangostana. Dichloromethane, ethyl acetate extract and the major xanthone (α-mangostin) were evaluated in vitro against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi and free trypomastigotes of T. brucei. The major constituent α-mangostin was also checked for antimicrobial potential against Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Bacillius subtilis, Staphylococcus aureus, Mycobacterium smegmatis, M. cheleneoi, M. xenopi and M. intracellulare. Activity against  P. falciparum (IC50 2.7 μg/mL) and T. brucei (IC50 0.5 μg/mL) were observed for the dichloromethane extract, however, with only moderate selectivity was seen based on a parallel cytotoxicity evaluation on MRC-5 cells (IC50 9.4 μg/mL). The ethyl acetate extract was inactive (IC50 > 30 µg/mL). The major constituent α-mangostin showed rather high cytotoxicity (IC50 7.5 µM) and a broad but non-selective antiprotozoal and antimicrobial activity profile. This in vitro study endorses that the antiprotozoal and antimicrobial potential

of prenylated xanthones is non-conclusive in view of the low level of selectivity.

Keywords: Garcinia mangostana; α-mangostin; in vitro; antiplasmodial; antileishmanial; antitrypanosomal

file:///C:/Users/Rafik%20Karaman/Downloads/molecules-18-10599.pdf

To view the full publication, please read the attached file.

Hoping this will be helpful,

Rafik

Dear Mr. Thiruventhan,

Attached pdf file entitled "Determination of total xanthones in Garcinia mangostana

fruit rind extracts by ultraviolet (UV) spectrophotometry" may help you to find out the phytochemical assay.

From which plant?

You can detect xanthophone in methanolic and petroleum ether extract by antimonytrichloride. It shows greenish blue color

Do first TLC and prepare potassium hydroxide reagent as follows dissolve 2 g potassium hydroxide in 5 ml water and make up to 50 ml with ethanol. Prepare a clear metanolic supernatant spot your samples onto TLC plate. You can detect the spots at 366 nm at various color ranges btw yellow to green, for cumarins green to dark blue.

Hi! According to the paper "Antioxidant Properties of Garcinia mangostana L (Mangosteen) Rind" written by Tjahjani et al, there are several tests or assays that can be used to detect xanthones. In this paper in particular, the researchers tested the presence of xanthones in mangosteen. They used the DPPH scavenging assay, Superoxide Dismutase activity assay, and total antioxidant status assay