Such compounds can be purified by C18 (see compound #6 here: http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN85_Purification_Strategies_for_Flavones_and_Related_Compounds.pdf )

The plant  material was extracted into methanol, and the methanolic extract was loaded onto a C18 flash column and eluted with a water/methanol gradient ( Meng, Y.; Krzysiak, A.J.; Durako, M.J.; Kunzelman, J.I.; L.C. Wright, J.L.C. Flavones and flavone glycosides from Halophila johnsonii. Phytochemistry, 2008, 69 (14), 2603-2608.)

Also look up Shiming Li as an author, as he has extracted various flavonoids from oranges- the references in the application not link may be a start.

Hi Thiruventhan

There might not be such fast isolation method because every process depend on time + material used.

I suggest you:

1. After crude extract of Orange peel, develop appropriate solvent system for Extract and hesperidin in TLC together (Chloform : MeoH may be good with gradient).

2. After finding proper solvent system, you can use either Preparative TLC in silica 120 lower.

3. Column with Sephadex LH20.

4. MS or HPLC will also good options but sample loadable amount is less. It might take much longer than you expected.

Hope it helps!

regards,

mathipi

Hi

you can see the link

https://www.researchgate.net/publication/273063971_Hesperidin_and_Hesperitin_preparation_and_purification_from_citrus_sinensis_peels

Article Hesperidin and Hesperitin preparation and purification from ...

you can isolate hesperidin easily by adding acetic acid to orange peel methanolic extract after defatting