What could be possible reason of higher Drug Concentration of Liposomal formulation stability samples over time?

Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...

25 January 2021 7,752 4 View

What is the advantage of DFT over tight-binding methods or vice-versa?

It might be a very basic question, but can somebody please put some light that what is the advantage of using Density Functional Theory (DFT) over tight-binding approximation or vice-...

31 August 2020 4,937 3 View

How the position of band inversion (near or away the Fermi level) affects the electrical transport and thermal properties in semi-metals?

Dear all, Can somebody please put some light on how the position of band inversion, near or away the Fermi level affects the electrical transport and thermal properties in semi-metals? Thanks in...

19 August 2020 5,202 8 View

My protein is a recombinant protein constitutively expressed in Pichia.Why am I getting a smear/ dragging of the loaded protein?

The protein (55KDa) has low contentration. It is purified via ammonium sulphate salting out and desalted with pD10 column. Will working with SDS-PAGE (10% resolving and 5% stacking), a drag is...

05 June 2019 4,216 14 View

How to perform band structure calculations with HSE + SOC in VASP?

Can Somebody please tell me that how to perform band structure calculations with HSE + spin-orbit coupling (SOC) in VASP? In normal HSE calculations, we perform SCF claculations with WAVECAR of...

07 January 2019 1,310 6 View

What is the meaning of parity of a band?

As we know, a band has partial contribution from all the orbitals of all the atoms, then how can we determine the parity of a band from these partial contributions? What is the physics involved in...

22 February 2018 5,256 8 View

Is there any theory which explains the origin of the existence of indirect band gap for a material?

As we know, Kronig Penney model explains the existence of band gap at the same 'k', i.e. direct band gap. However, there is nothing like indirect band gap in KP model. Then, how can we explain the...

02 May 2017 7,619 3 View

What are High symmetry points in a crystal?

12 February 2017 6,848 7 View

PSI Short form Abidin scoring?

Can anyone please guide me about how to interpret the subscale scores for the Parenting Stress Index (Esp the parental Dysfunctional subscale). Any guidance will be much appreciated!!!

18 January 2017 2,828 3 View

Any ideas of how to run a power analysis to determine sample size for a within group study?

Hi I am planning to run a within group study (collecting data from the same participants thrice). The questionnaires I am using are the BRIEF COPE, PSI (Parental stress index) and PSS (perceived...

13 November 2016 3,357 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View