Researchers in the field of wheat development and improvement will already know that there have been 2 confirmed Sucrose Transporter Genes (SUT1 and SUT2) that have been identified and confirmed in Triticum aestivum so far. I am working with these genes and 3 others (SUT3, SUT4, and SUT5) from rice as i have found similar sequences in Triticum. Am very new to crop biotechnology field and currently in the process of designing my first set of primers. Later in my work, am planning to do yeast 2 hybridization and co-immunoprecipitation. I have a hypothetical pathway of gene certain gene interactions with sucrose that i would like to work towards proving. So my question is, "is there any particular experiments that i could do whereby i can show sucrose interactions with multiple genes simultaneously or is it 2 genes at a time?" Also as I mentioned i am quite new in biotechnology, so any advice on experimental skills or wheat growth or good lab practices would be highly appreciated.
Thanking you in advance.
Dee
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