My pollutant include dyes. GCMS sample should be devoid of water, i am not getting how to take it in methanol solvent after degradation?

My pollutant include dyes and drugs. GCMS sample should be devoid of water, i am not getting how to take it in methanol solvent after degradation product complete. I have taken the sample and...

01 November 2020 5,840 3 View

What are the basis for determining assessment factors during chemical risk analysis?

I have EC50 and LC50 values and wanted to calculate PEC for different species in water. I have currently considered acute risk with an assessment factor of 100 based on a previous similar...

12 July 2020 4,562 2 View

Is Hemispheric Brain Dominance a current research topic in neuroscience/psychology ?

I want to start research regarding Brain dominance and some concepts of positive psychology. That is why I ask whether or not it is a research area that is relevant now. If not could you please...

09 March 2020 6,857 5 View

Storing tissue in Agarose for 24 hrs ?

Dear All, I want to perform tissue section on different kinds of tissues like brain, muscle tissue,etc. I would like to store my fresh tissue in agarose for 24 hrs/over night before sectioning...

16 February 2020 878 2 View

How to generate PSF and perform Deconvolution?

I have recently started learning about deblurring images using deconvolution. I have a stack of images (in .tif format) of size 873 MB (1392 x 1040) and a total of 301 images in a single .tif...

04 February 2020 5,866 1 View

What is the Difference beetween crystallite size calculated by PXRD and crystal size observed by SEM analysis ?

let us consider some metal oxide particles, which shows cubic shape with some specific size of a singe cube in SEM image. now if we calculate crystallite size of the same with its PXRD data, then...

09 October 2019 6,326 5 View

How to differentiate T. elliptica, T. tomentosa, T. crenulata and T. alata? Different or synonymous?

Going through every literature and floral publication, they have their own theories, some say different species some consider synonymous to each other. There is a need of Key to Terminalia species...

08 September 2019 3,029 3 View

Does an elastomer expand the same way every time it's stretched?

Say there is an elastomer strip of length L, width W and uniform thickness -T. All other parameters like material density, temperature are constant. I mark this strip with N points starting...

25 October 2018 740 3 View

Can we leverage CIM (Common Information Model) framework for using big data in power industry?

Can we leverage CIM (Common Information Model) framework that is already in place through IEC standards (IEC 61970, 62325) for the big data use cases in utility industry??

13 September 2018 4,762 1 View

Can a Bioanalyzer be used to test for DNA fragmentation?

I am working on a project using DNA from fairly old tissue specimens (>30 years). We will be extracting DNA from these samples for sequencing, but we want to see how fragmented the DNA extracts...

31 January 2018 3,636 1 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View