Are there any papers citing formation of dorsal ruffles at room temperature rather than specifically at 37 deg.
Thanks...
I am looking to simulate real-world landslides in a laboratory setting and measure parameters such as volumetric water content, pore water pressure development, seepage patterns, and ground...
23 June 2024 8,340 2 View
Trying to estimate the DBTT temperature for A572 & RHA steel based on alloying composition.
20 May 2024 8,060 8 View
Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...
13 May 2024 4,935 7 View
We have expressed and purified the recombinant viral protein found in the insoluble fraction. Please suggest some methods or reagents to solubilize the protein.
23 January 2024 9,327 5 View
Suppose I am making a solution of a particular concentration and I am doing it with step wise dilution from a stock solution. If I use a same volumetric flask for 2 time for the consecutive...
14 January 2024 1,976 10 View
I have been able to use the Kraken2 pipeline for analysing the nanopore reads and derive a KronaPlot for metagenomics. Since KronaPlot also gives a list of reads from a sample that is associated...
21 November 2023 1,989 5 View
Taxonomy of Brachymeria species
14 September 2023 3,409 2 View
I intend to use Silvaco TCAD for the CNFET simulation but from what i could gather Silvaco does't support CNT by default. Thus if someone could guide me in this regard if whether i would be able...
23 August 2023 569 1 View
🔬 Calling Fellow Researchers: Share Your Insights on AI and Future Work Hey researchers! 🧠📊 Exploring AI's impact on future work and job displacement. Looking for your insights, case studies,...
06 August 2023 4,304 2 View
how it gonna happen and Is restriction enzymes can functionally manipulate bacterial system ?
18 June 2023 2,249 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View