What do you mean by new? I you have tried all of the standard methods for detecting protein then I would think you would come up with some difference between a treated cell/tissue and controls. If you mean how do I detect protein expression in general then it will be driven entirely by your budget. Let us know and lets go from there.

thank you for your reply,i am using Western blot ,,,,,

you can try phosphokinase array kit for instanse produced by R and D.

Sumia Essid wrote:

> I would like to ask if there is any new method to investigate the

> intracellular protein expression such as MAP Kinase ,

There's a method with fluorescence.

http://www.sciencedaily.com/releases/2007/12/071213124908.htm

Other:

http://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.1000041

http://www.innovations-report.de/html/berichte/biowissenschaften_chemie/bericht-106200.html

http://www.innovations-report.de/html/berichte/biowissenschaften_chemie/bericht-100238.html

http://www.princeton.edu/main/news/archive/S26/81/07Q04/

Regards,

Joachim