@ Joseph, please have a look of the attached file.

Hello,

please have a look at the following articles:

https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400041

Article Endophytic bacteria: Optimizaton of isolation procedure from...

https://www.researchgate.net/post/What_is_the_best_method_to_isolate_endophytes_from_leaf_samples_Can_anyone_plz_quote_a_good_research_article_for_the_same

Best regards

Thank you Dr. J. C. Tarafdar ...

Thank you Dr. Desiderio ....

Bacterial endophytes can be isolated.kindly refer to my paper for the methods.recent publication of bacterial isoletes from Cissus sp.still if you have a querry ,i can definitely help you.

The direct answer to your question is YES. I agree with the recommendations above. I would like to point out that cultivation conditions, as well as used microbial broths, are very important during the isolation of bacteria from natural samples because many bacteria present in nature are not cultivatable under common laboratory conditions.

Best regards

Vit

Thank you Dr. Vit Mateju

Thank you Dr. Uma Gowrie Sezhian