How to estimate nares to LES distance from demographic measures in adults?

There are some formula for estimating nares to lower esophageal sphincter distance in infants based on height. I'm looking for some data in adults to estimate such distance based on gender and...

31 December 2017 1,190 5 View

How to calculate sample size for non-inferiority test using G*Power?

Is it possible to calculate sample size for non-inferiority/superiority tests using the G*Power software?

03 April 2017 6,890 1 View

What band-pass filter is appropriate for continous blood pressure wave analysis?

We use the analogue output of the Portapres device for monitoring continuous blood pressure. Is band-pass filtering necessary for such blood pressure wave analysis? What should be the low/high...

10 November 2016 9,852 15 View

What does it mean when, in the repeated measure analysis, the time x group interaction is significant but between-subject contrast is not significant?

In the repeated measure analysis of the the effect of drug A compared with drug B on the variable C which was measured at times 0, week 2, and week 6, the results were as follows: Test of...

31 December 2014 7,441 13 View

What could be the mechanisms for vitamin D deficiency associated with radiation-induced intestinal injury ?

In a prospective study on patients with pelvic malignancies undergoing pelvic radiotherapy, we found an association between vitamin D deficiency and severity of radiation induced proctitis. This...

11 December 2014 1,496 8 View

What are the Pros and Cons of measuring 1,25(OH)2D in contrast to 25(OH)D to investigate the immunomodulatory effects of vitamin D?

10 November 2014 7,355 7 View

Can anyone recommend a good questionnaire for evaluating coping in cancer patients?

We are looking for a questionnaire to evaluate coping in cancer patients. We look for an instrument designed specifically for cancer patients.

02 March 2014 3,124 3 View

As independent factors, should anxiety and depression be included in a linear regression model separately or simultaneously?

In studying factors associated with sleep in patients with chronic medical conditions we found an association between different factors including anxiety and depression with sleep quality. To...

31 December 2013 7,349 33 View

What is the minimum acceptable item-total correlation in a multi-dimensional questionnaire?

In linguistic validation of some multi-dimensional questionnaires for our population (with 26 to 34 items and about 5 sub-scales), we encountered some questions: What are the minimum acceptable...

10 November 2013 5,304 28 View

Association of BMI and treatment response in rheumatoid arthritis - can anyone help?

Recently we have conducted a prospective study to evaluate if BMI is associated with treatment response to a combination DEMARDs therapy. We found an inverse association between BMI and disease...

09 October 2013 730 14 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View