I want to screen soil samples for azotobacter and azospirillium, so is there any plate or chemical assay to quickly screen samples, after that when I identify it as any one above them, then please suggest any molecular or other method to identify the strain of the same..
Please have a look at these useful links.
https://www.researchgate.net/publication/237194371_Isolation_of_Azospirillum_lipoferum_from_the_rhizosphere_of_rice_by_a_new_simple_method
https://www.researchgate.net/publication/273521070_Isolation_and_identification_of_Azospirillum_isolates_from_different_paddy_fields_of_North_Bengal
https://www.researchgate.net/post/What_is_the_best_method_for_isolation_and_identification_of_bacteria_from_soil
https://www.researchgate.net/post/How_can_we_identify_Azotobacter_from_a_soil_sample
https://www.researchgate.net/publication/227252152_Isolation_and_characterization_of_Azotobacter_and_Azospirillum_strains_from_sugarcane_rhizosphere
https://www.researchgate.net/publication/222610140_Isolation_and_characterization_of_Azotobacter_chroococcum_from_the_roots_of_Zea_mays
Article Isolation of Azospirillum lipoferum from the rhizosphere of ...
Article Isolation and identification of Azospirillum isolates from d...
Article Isolation and characterization of Azotobacter and Azospirill...
Article Isolation and characterization of Azotobacter chroococcum fr...
Characterization of Azotobacter
Direct microscopy investigates was used for pure isolates of Azotobacter. It was found that the microbe is gram negative, large in size, oval or cocci, single or in pairs according to Bergy's Manual (1974) and Becking (1981). Identifying testes were carried out including (IMViC) tests, it was positive in indole test and negative in methyl red , voges proskauer , citrate , gelatin hydrolysis catalase , cellulase , pectinase and amylase activities .Nitrite formation and denitrification tests were carried out by the method described by Neyra et al., (1977) in addition to nitrogenase activity according to (Hardy et al., 1973) and litmus as described by Drews (1983) and Sussmulh et al. (1987)
Characterization of Azospirillum
Pure isolates being short rods of spinning motility gram negative, producing alkalinity in growth culture .The ability of Azospirilla to use glucose a sole carbon source for growth was described by Tarrand et al. (1978), positive in production of acid and gas, requirement of biotin for growth , catalase production and reduction NO3 to NO2.
Dear
Protocol of Mazinani can help. Different Methods for Isolation and Preliminary Identification of Azotobacter. See the link below.
http://ijasr.srbiau.ac.ir/article_5588_a1354878f5f9880bc48153c134ccbd42.pdf
Thank you all for your kind response.
But I want to produce azotobacter and azospririllum cysts in minimal time, how should I do that?
Please check the below given link and PDF attachments.
https://www.researchgate.net/post/Is_it_possible_to_induce_cyst_formation_of_Azotobacter_after_fermentation_in_laboratory_bioreactor_in_liquid_medium
Please have a look at this link also.
https://www.researchgate.net/publication/259756879_Azospirillum_and_its_Formulations_A_Review
Article Azospirillum and its Formulations: A Review
Kindly have a look here..
http://www.eulc.edu.eg/eulc_v5/Libraries/UploadFiles/DownLoadFile.aspx?RelatedBibID=ODIyYzZmNDUtOTQ5YS00MjQyLWIyOTItMDAxYjhmYTlhYjFkX2l0ZW1zXzEyMTQwMTkyXzExNzg4NDAwX18=&filename=93-104.pdf
See through this too..
https://www.jstor.org/stable/24125109?seq=1#page_scan_tab_contents
See, this also..
http://ijasr.srbiau.ac.ir/article_5588_a1354878f5f9880bc48153c134ccbd42.pdf
Some more info..plz.
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300003
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