Dataset: Orbitrap velos CID MS/MS spectra ?

Hi, I want the MS2-CID spectra from orbitrap velos mass spectrometry of organism: human (Mod: C+57) for testing my peptide identification algorithm developed. Large set of orbitrap velos dataset...

16 November 2020 8,261 1 View

Does infection duration vary?

I would like to know the ideal duration of HSV infection in Vero cells, as this is affecting the antiviral assays. How can we maintain the viral infective rate while conducting a battery of assays?

21 June 2020 3,880 5 View

Is there any open source tool to identify peptide from peak list input file using Sequest & Mascot algorithm?

I have an input file containing precursor mass and peak list (m/z and Intensity) of tandem mass spectrum. The file contains multiple spectrum data. I need to identify peptide using sequest and...

09 April 2019 8,354 4 View

Why cancerous cell line is used for T 2 Diabetes study eg. HepG2 ?

If HepG2 cells are used for diabetic studies is this necessary to show/perform the same experiment in normal cell lines.

01 January 2019 5,258 4 View

How can I interpret the negative value of regression coefficient in logistic regression??

I am running a logistic regression by using dichotomous dependent variable and five independent variable. I found one of the independent variable is getting -ve regression coefficient. 1) What...

07 November 2017 4,464 9 View

Experimental research design

09 July 2017 6,099 8 View

How to generate a incommensurate Supercell POSCAR for VASP?

Hello All, I have a 1x1x1 POSCAR, I need o make a 3 square root 3X1X1 supercell. I understand VESTA cannot make it, are there online tools available for generating this? I have attached my...

25 October 2016 8,267 4 View

Can anyone suggest from where I can get Microgrid data, such as nodes load, impedance etc ?

I'm working on Microgrid load management.

17 October 2016 8,272 5 View

Is atmospheric correction mandatory for chlorophyll estimation from Landsat images and which is best method of atmospheric correction?

I am trying to estimate chlorophyll-a by using Landsat/ MODIS image. So I want to know atmospheric correction for the above analysis is required or not? There is so much technique for atmospheric...

08 March 2016 9,488 2 View

How effectively can the anti diabetic potential of an active component of a plant extract be established in cell lines which mimic diabetes?

Primarily, Type 2 Diabetes Mellitus

26 December 2014 412 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View