Is there any IgA, IgM, IgG, Kappa, and Lambda clone suitable for mice tissue?

13 March 2020 0 8K Report

As title. Need to do kidney study in mice. Anyone has successful experience in immunofluorescence study, IgA, IgM, IgG, Kappa, and Lambda, in mice kidney?

Badges
Science method

More Peir-In Liang's questions See All

Can diamond be grown using molecular beam epitaxy?

22 July 2024 9,755 2 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

Who can help me to get the PDF file of this paper?

Yuen P.H. 1965. Some studies on the taxonomy and development of some Rhabdisoid and Cosmocercoid nematodes from Malayan amphibians. Zoologischer Anzeiger, 174, 275–298. Thank you very much for...

07 May 2024 9,971 0 View

Who can help me with the following three papers?

(1) SHARPILO, V. P. 1961. New nematode Spauligodon saxicolae nov. sp. Parasite of the scaly lizard Lacerta saxicola Evers. Trudy Ukrainskoe Respublikanskoe Nauchnoe Obshchestvo Parazitolohov 1:...

02 May 2024 4,116 0 View

Who can help me with the PDF file of this monograph?

Sharpilo, C. P. (1976). Parasitic worms of the reptilian fauna of the USSR: Systematics, chorology, biology. Moscow: Naukova Dumka, 287 pp (In Russian). Thank you for all your kind helps!

02 May 2024 2,013 4 View

Seeking Scanned or Digital Copies of Specific Academic Books?

Hello everyone, I am currentlyseeking scanned or digital copies of the following books: Angelika Linke (1985): Gespräche im Fernsehen: eine diskursanalytische Untersuchung. Bern: Peter...

20 April 2024 9,178 0 View

What is the method of whole blood stimulation for Flow cytometry (FACS)?

Hello. I am a senior graduate student preparing the ICS protocol. Despite not being in the relevant major, I am experiencing difficulties in preparation and therefore I have a question to ask....

18 April 2024 7,796 0 View

What's the Role of Live Stream influencer Marketing on Consumer Brand Engagement?

What's the role of Live Stream influencer Marketing on Consumer Brand Engagement and Brand Preference?

16 April 2024 229 1 View

Hi everyone, I would like to ask what concentration of ammonium nitrate do I need to add?

I need to add NH4NO3 and KH2PO4 as nutrients for seagrass growth to the artificial water which is made from sea salt, but I only know the previous data and don’t know how to calculate how many...

25 February 2024 778 7 View

Conjunction of autocorrelated residuals and factor analysis/SEM in psychometrics?

Basically most studies of "structural equation modelling + time-series data" focus on longitudinal datasets (e.g., (residual) dynamic SEM). However, for a questionnaire survey or measure at a...

21 November 2023 7,979 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition of \lambda?

For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?

23 July 2024 8,008 1 View

Not able to give GAFF forcefield to my ligand?

I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...

15 July 2024 7,323 1 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to reduce the material coming out of the torque rheometer?

A physical mix was in vitro evaluated on a torque rheometer. The composition was predominantly recycled polypropylene (PP), yet the melt flow index (MFI) was found to be excessively high....

25 June 2024 10,154 0 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Depletion igg from serum?

Hi everyone, I wanna get a suitable protocol for depletion of IgG and other immunoglobulins from serum, without kits or chromatography but with chemical methods like ammonium sulfate. Can anyone...

10 June 2024 9,523 2 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

How is it possible to model a protein that consists of several subunits using a Modeller?

Good day, I am trying to model the GABA A receptor, with different subunits. I selected the templates with the highest percent of identity, but as a result of the modeling I got a protein with...

02 June 2024 5,489 2 View