Hello

# Human p16INK4a / CDKN2A Antibody / R&D system 

# Human p16ink4a--cdkn2a-antibody / clontech

# Ab for p16ink4a / abcom 

it's agood choices

Restart your experience and check of the staining steps .

Good Luck

Hi Saleh,

Did you tried any of these for immunofluorescence? 

Hi Lin,

Did you check the recent Molecular Cell paper by Dorota Skowronska-Krawczyk? I think they have used qPCR for quantification of P16INK4a. I am pretty sure that they would have tried some of the available antibodies, therefore there may not be a good antibody. Perhaps you should directly contact them ask whether they have tried any of the antibodies.

Are you working on retinal slices or whole-mounts?

best wishes,

Refik

Hi Refik,

Thanks for your reply. I'll do the staining on vertical sections as well as flat-mounts. I also did qPCR. I tried the antigen retrieval method today before proceeding immunostaining using the ab from abcam. It worked well. Thanks for your help. 

Lin