Actinobacillus actinomycetemcomitans is a member of the family Pasteurellaceae and a major causative agent of periodontitis. While several genera from this family are known to be competent for transformation, A. actinomycetemcomitans has yet to be fully characterized. Here we show that the competence of A. actinomycetemcomitans is remarkably similar to that of Haemophilus influenzae. In addition to having a similar frequency of transformation as H. influenzae, A. actinomycetemcomitans competence could also be induced at least 100-fold by cyclic AMP, suggesting that, as in H. influenzae, at least some competence genes are regulated by catabolite repression. Even more intriguing was the discovery of a putative A. actinomycetemcomitans DNA uptake signal sequence (USS) virtually identical to the USS of H. influenzae. Moreover, we provide evidence that this sequence functions in the same capacity as that from H. influenzae; the sequence appears to be required and sufficient for DNA uptake in a variety of assays. Finally, we have taken advantage of this system to develop a simple, highly efficient competence-based method for generating site-directed mutations in the wild-type fimbriated A. actinomycetemcomitans.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC135135/

Apply your transformation protocol to a mutiple independently isolated clinical isolates. Likely then to find one that is naturally transformable. 

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Please find links below.

https://www.ncbi.nlm.nih.gov/pubmed/11375171

https://www.ncbi.nlm.nih.gov/pubmed/11244078

https://www.ncbi.nlm.nih.gov/pubmed/7968924

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068243/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4073975/

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