Using data from ITS massive parallel sequencing, is it possible to do an silico functional inference similarly to what is it done to 16S rRNA gene using PICRUSt or Piphillin?
These softwares are used for bacterial functional inference, IS there available any similiar software for fungi molecular markers?
Thank you in adavance!
Marta Alves Yes! In the way such kind software packages are only available for 16S RNA gene yet. And even they are giving a very rough scenario about the actual functional profiles.
Nevertheless, you can use few web based tools available on Ensemble fungi and EBI/NCBI for your purpose.
Ajit kumar Roy As always you dont read questions and post absurd answers, which dont really contribute knowledge to the discussion.
Hi Marta,
FUNGuild is a free, user-friendly tool that can be used for assigning fungal OTUs to their ecological guild (ectomycorrhiza, saprobe, pathogen, etc). Although it doesn’t predict the metagenome as the PICRUSt, you can use it to find functional traits based on ITS high-throughput sequencing data.
Hope this helps you.
Diogo
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