I'd like to perform an extraction followed by various chromatography analyses. I am looking for protocols related to this issue
Plant tissue culture system are effectively used in commercial production. Of those, African violet is one of the best example for cost effective production. After emergence of new tecnologies...
31 May 2019 574 5 View
Photolysis is one of the critical step for photosynthesis as an electron source.Do you know any plant that undergoes photosynthesis by passing PSII, photosystem II, (or without it), generating...
28 January 2017 8,296 14 View
Do you have an experience of MAP used for hardening steps of vitroplants especially in woody plants? In some cases, MAP is highly effective for root induction as compared to the auxins. I need...
17 November 2016 373 4 View
What do you think of the concentrations used in tissue culture papers? For example effects of 0.5 mg/L IAA or IBA on root formation.... Is this a comparable issue? Because this also means 2.9 uM...
23 December 2014 9,892 8 View
I would like to estimate stevioside and rebaudiosid A content in dried leaf materials using HPLC. Could you reccomend me an efficient extraction protocol and appropriate HPLC conditions for that?
15 August 2013 7,621 4 View
I am using Dionex ultimate 3000 fraction collector and try to isolate some target peaks. But, it seems tiring/boring job when you cannot acheive it exactly. Do you know tips that makes it easier?
21 May 2013 1,120 5 View
There are plenty of papers involving phytochemical analysis for certain plant species. Moreover, target compounds isolated different plant organs produced in vitro or collected from different...
03 April 2013 962 3 View
Callus cultures in plant regeneration systems are sometimes useful for conservation. Moreover, they are capable of new variants. What do you think about secondary metabolite production from callus...
24 December 2012 4,513 7 View
DAD is almost twice expensive than UV dedector. Is there any significant difference in analysis (i.e. natural compounds in plant extracts) when comparing these two?
15 October 2012 1,571 9 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View