I'd like to perform an extraction followed by various chromatography analyses. I am looking for protocols related to this issue
I would like to estimate stevioside and rebaudiosid A content in dried leaf materials using HPLC. Could you reccomend me an efficient extraction protocol and appropriate HPLC conditions for that?
15 August 2013 7,459 4 View
There are plenty of papers involving phytochemical analysis for certain plant species. Moreover, target compounds isolated different plant organs produced in vitro or collected from different...
03 April 2013 854 3 View
Callus cultures in plant regeneration systems are sometimes useful for conservation. Moreover, they are capable of new variants. What do you think about secondary metabolite production from callus...
24 December 2012 4,385 7 View
DAD is almost twice expensive than UV dedector. Is there any significant difference in analysis (i.e. natural compounds in plant extracts) when comparing these two?
15 October 2012 1,350 9 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View