We are growing microalgae Haematococcus in open ponds. After two days of thunderstorms and lightening we observed cells were started showing signs of lysing. Could electrostatic field of lightening play any role here?
I also found damaged Trachelomonas cells in ponds usually after raining. I think it may not be due to lightening but because of change pH or osmatic concentration of water.
Generally osmotic pressure (hypo) is used to lyse the cells.
Agree with Mallesh Bandi. Check the pond pH after such kind of event. And also you should have everyday pH values in ponds. Next thing...check the samples from pond surface, mid, and bottom. if you can count the damaged cells from each samples. Compare it with normal day samples.
Rain with lightning brings acidic water. So pH change is possible.
Rain drops disturb to the pond surface and may brake the cells.
If your pond is mechanically mixed one...this factor is not accounted....
If the pond is stagnant, rain allows it to mix. Usually stagnant pond bottoms have different chemical conditions than other layers. It may lead to osmotic changes in cells.