Can I use DNA silver staining procedure for protein saining after SDS-PAGE
Silver staining used for small quantity of sample. For ex. In protein staining, some proteins very low expression and amount of protein is less so for this type silver staining can be used
No there is no big difference in protocols, but there are protocols optimized for every biological molecule, look here:
http://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.330.html
I used a 1.5mm thickness gel for western blot at 100v for 90min, but the transfer did not complete. Is it necessary to increase the time for an effective transfer?
01 February 2013 2,971 6 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View