Marcin Michalik

3 Questions 23 Answers 0 Followers

Questions related from Marcin Michalik

How behave detergents in CD spectra in temperature range?

Hi, is it fine to use protein refolded in LDAO or SB-12 detergent for thermal stability experiment with CD spectroscopy? CD spectra as a function of temperature. Will LDAO or SB-12 have influence...

02 February 2016 3,692 2 View

Can anyone help with refolding after heat denaturation?

Is there any influence of heat denaturation on outer membrane proteins (bbarrel)? Except defolding of course, something like permanent structure change? If protein will be expressed as inclusion...

12 December 2013 434 8 View

Protein changes in Urea

I want to ask if storing proteins in Urea causing any changes in structure. If yes - how often? How to prevent it or check if there are any changes? What time and temperature of storage is safe...

06 June 2013 8,764 24 View