.In my results the slope of curves got from Microplate reader is seems less (less than 1) and negetive some times. What is the reason for that?

In my research I'm enumeraring the florescence intensity (FI) Vs E-coli concentration in river water. I measure FI by Tecan I control 200 pro and E-coli by Membrane filtration.

03 January 2021 2,991 3 View

Is Thermodynamics not able to get the heat transfer rate value?

In the first lecture of heat transfer course, generally it is told that with Thermodynamics, it is not possible to get the heat transfer rate of a process. But in steady flow energy equation (time...

29 February 2020 9,700 9 View

What are the signal processing techniques to extract a low amplitude single/ multiple source signal from the received noisy signal?

I am having a received low SNR signal. It can contain upto 3 sources of signal, each of which has a different dominant frequency (say 1GHz for source 1, 1.5 GHz for source 2, 2 GHz for source 3)....

28 September 2018 1,722 11 View

Why do academic research outcomes hardly inform development policy practice in Africa? How can we effectively address this institutional inaction?

I am particularly worried about development challenges most African countries face despite their human and natural resources potentials. Moreover, International organisations have funded several...

13 June 2018 4,334 19 View

I'm trying to stain cancer cells grown on gel. I'm not sure whether I need to fix my cells with 4%PFA or ethanol before staining?

I am trying to stain #ovarian cancer cells grown on gel with ethidium bromide and calcien. I am not too sure whether to fix my cells with 4% PFA or ethanol before staining. Any suggestions will be...

06 February 2016 2,397 4 View

Is Grid Independency Test (GIT) a must for complex numerical models?

17 December 2014 7,259 20 View

Why does my citrate buffer turn milky during immunohistochemistry?

For my IHC protocol, I am staining for beta-catenin in ovarian sections during antigen retrieval. My samples are heated in citrate buffer for 45 mins. I have also read that signs to show that your...

08 December 2014 1,939 3 View

How to find the physical properties of a mixture of 2 or 3 salts with known proportions?

19 November 2014 9,137 5 View

Does XRD output data has any significance in the field of Thermal Energy Storage (especially Phase Change Materials)?

With the output of XRD, we can get any information relevant to Phase Change Materials of Latent Heat Storage?

19 November 2014 5,334 3 View

Is it possible to define 2 materials for a single domain in COMSOL?

I am working on flow through porous media. Flow characteristics can be solved using Brinkman Equations physics and Heat Transfer characteristics can be solved using Heat Transfer in Porous Media...

18 November 2014 3,932 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View