I cannot detect Parp protein after total protein isolation?

I cannot detect Parp protein after total protein isolation Hello everyone; I cannot detect the Parp protein after total protein isolation. I can see my cells die morphologically after a novel...

18 July 2019 2,593 2 View

At what stage should I use the DNAse I reaction protocol?

Protocol content (NEB DNAse I reaction Protocol) : RNA: 10ug DNAse reaction buffer 1X 10ul DNAse (Rnase free) 1ul Nuclease free water 100 ul My question is; if I use this protocol after isolating...

11 January 2019 1,249 3 View

GNSS Carrier phase Single difference residuals?

Dear all if I have the SD carrier-phase measurements how can I compute the SD residuals? Do i need to calculate the SD residuals before fixing the integer ambiguity?

10 April 2018 7,001 2 View

When is it a good time to consider relative risk reduction and when is it a good time for attributable risk reduction?

The above question is a prevalence question. If the prevalence of disease is high (and assuming that the disease is serious), we would consider ARR (=Risk in unexposed - Risk in treated ) versus...

06 May 2014 9,720 2 View

Is GC content important when designing oligonucleotides for transfection?

Is GC content important when designing oligonucleotides for transfection? Is this a problem for oligonucleotide-based transfection, where the dna region I will design oligonucleotide is very rich...

01 January 1970 988 3 View

How to release matter from genipin crosslinked polymer?

01 January 1970 4,805 4 View

Do you need specially designed vectors (such as addgene) for Crispr / Cas9 ?

Do you need specially designed vectors for Crispr / Cas9 ? If so, why? Can't I clone the gene into the restriction site of a vector I have?

01 January 1970 6,370 2 View

What do you think about the state of the cell?

HEK-293T is becoming this for a while after being in culture. (20X magnification) Other cells in the culture have no such problem. Can you describe this morphology?

01 January 1970 2,913 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View