Is there any cheaper alternative for blue dextran, in order to calculate void volume in gel filtration chromatography?

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Amog P Urs

Void volume is equivalent to approximately 30% of the total column volume (packed bed).

Bruce Levison

If you are up to preparing a void volume standard try the macromolecular complex of Prussian Blue. The fist link US patent 3694149 gives the preparation of the material and the second link US patent US3885921 gives its purification.

http://www.google.com/patents/US3694149

http://www.google.com/patents/US3885921

Greg Cote

Blue dextran is easily made by the methods described in this paper.