Is there any cheaper alternative for blue dextran, in order to calculate void volume in gel filtration chromatography?

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Bruce Levison

If you are up to preparing a void volume standard try the macromolecular complex of Prussian Blue. The fist link US patent 3694149 gives the preparation of the material and the second link US patent US3885921 gives its purification.

http://www.google.com/patents/US3694149

http://www.google.com/patents/US3885921