Is there any alternative for glycogen solution in DNA precipitation?

19 February 2020 1 2K Report

Hi I am a master student working on Parrafin-embedded tissue to extract DNA and I could't find glycogen 20mg/ml solution used in the DNA precipitation an I am wondering if there is any alternative.

Thank you for your help

S M Zia Hasan

You can use this procedure:

Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample
Mix, and store at -20°C for at least 1 hour to precipitate the DNA
Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15-20 minutes
Pour off the ethanol and wash the pellet twice with room-temperature 70% ethanol
Allow the DNA pellet to air-dry
resuspend the DNA in a suitable volume of sterile TE buffer or distilled water
Source: https://www.qiagen.com/ca/resources/faq?id=5d591b8b-968a-4a17-849f-9d0f719b40af&lang=en

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