Ayyoub Kihel

5 Questions 3 Answers 0 Followers

Questions related from Ayyoub Kihel

What is the interval of mouse bêta actine experience cycle (cq)?

Hello, I am doing qPCR experiments on RNA extracted from mouse spleens and ganglia. At first I found that beta actin is expressed from 28th cycle. I tried to lower the hybridization temperature...

07 August 2023 8,092 3 View

Splenocytes stimulation for inflammatory cytokine measurement?

Hello, I'm trying to stimulate murine cytokine to induce inflammatory cytokine production such as IL-18 and IL-1beta. Can LPs be used as a stimulant? If so, what is the recommended...

18 May 2023 549 3 View

Is there any alternative for glycogen solution in DNA precipitation?

Hi I am a master student working on Parrafin-embedded tissue to extract DNA and I could't find glycogen 20mg/ml solution used in the DNA precipitation an I am wondering if there is any...

19 February 2020 1,658 1 View

Monocyte isolation by ficolle separation and adhesion selection?

Hello. I am a student in master cycle, I would like to study the activation of the inflammasome in vitro in human monocytes, we do not have a monocyte isolation kit so I use the classical...

01 May 2019 9,916 7 View

Is it possible to adapt the RPMI-1640 medium for the cultivation of HEK-blue cells?

Hello I am a master student, I want to work with HEK-blue cells (cells derived from transfection of hek 293 cells, these cells are developed by invivigens). According to the supplier's...

16 February 2019 2,522 2 View