Why have peaks at 590 and 798 cm-1 appeared in the IR spectrum of biogenic carbonate deposits?

04 May 2015 1,027 9 View

Is there anyone with research experience and interest in investigation of biogenic calcium carbonate polymorphs?

02 March 2015 7,850 3 View

Comparing the XRD patterns of mixtures containing CaCO3, how can I just roughly prove that one sample contains more aragonite than another one?

I have the XRD patterns of two samples which mainly contains calcite and aragonite (S1 and S2, see the attachments). How can I just roughly say that S2 has more amount of aragonite than S1?

01 February 2015 1,393 12 View

How can I find the urea concentration within a urea hydrolysis process?

01 February 2015 2,751 3 View

What does it mean when a reaction has a rate constant greater than one (K>1)?

I recorded the concentrations of a product during a reaction. There was an enzyme producing bacteria as catalyzer. Then I plotted the concentration changes versus time and fitted a modified...

31 December 2014 9,073 9 View

Why is a sample contained Calcite identified as Nitratine by XRD analysis?

I analyzed a sample contained calcite using XRD. The pattern attached. It was identified as nitratine by the related software searching the database of XRD patterns. As nitratine is isostructural...

31 December 2014 5,513 10 View

How much a fit model with the following statistics is reliable?

How much a model with: - Model p-value < 0.05 - Lack of fit p-value > 0.1 - R2=0.99, Adj. R2= 0.99, Pred. R2=0.99 - normal probability plot attached (residuals do not follow a straight...

09 October 2014 5,187 2 View

Is anyone familiar with full factorial design?

I did a 2-level full factorial design with 3 factors in order to find the significance of each factor and their interactions on a response. After ANOVA on non-transformed response, I found no...

08 September 2014 331 11 View

Generalized linear model or Least squares model?

I did the following full factorial experiments to find the significance of variables and fit model. I analyzed the data using the standard least squares model and generalized linear model (normal...

08 September 2014 2,582 6 View

How can I analyze a full factorial design with some undetectable responses due to interaction between factors?

08 September 2014 7,333 5 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View