Is there any additive dye which has no influence on bacterial activity?

More Hamed Khodadadi T.'s questions See All

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Thermal and mechanical properties of concrete and steel after cooling?

How do the thermal conductivity, specific heat capacity, plastic strain, critical compressive displacement, and thermal expansion of both hot-rolled steel and concrete change after cooling based...

15 February 2021 1,385 5 View

Cross-sectional area and mesh sensitivity in lattice modeling of RC structural elements?

In lattice modeling of RC structures, what is the mathematical concept behind choosing the cross-sectional areas for the simulation of, for instance, beams? Besides, seemingly, simulating the...

26 January 2021 2,638 2 View

What is the best word embeddings method should I use?

Hi dears If I want to do supervised multi-classes classification of specific domain such as history, using one of the deep learning techniques. Which is the best word embeddings method should I...

22 January 2021 1,290 5 View

What is the best lighting design for conservation?

In the museums, the sensitive organic materials need to be displayed under 50 lux or less and aways from any UV or IR. LEDs still have a little amount of UV. What is the better option? Is it...

20 January 2021 5,965 3 View

Fracture energy of concrete at sub-zero temperatures?

Both tensile and compressive strengths of concrete increase with the decrease in temperature. With this in mind and considering that fracture energy is in direct correlation with the so-called...

18 January 2021 8,778 4 View

Is it useful in texts classification to use the keywords expansion method for long-text examples in the training process for deep learning classifier?

Hello, Is it useful in texts classification to use the keywords expansion method for "long-text examples" in the training process for deep learning classifiers? Will it increase the efficiency of...

16 January 2021 9,291 3 View

Why the fracture energy of concrete increases with the rise in temperature?

Given the degradation of mechanical properties in concrete under elevated temperature namely, tensile strength, modulus of elasticity, compressive strength, etc., what is the justification behind...

15 January 2021 3,651 4 View

Is It useful to use text Pre-processing techniques (Stop Words Removal, Stemming) in the sentiment analysis classification?

Hello, I read some papers they used Pre-processing steps with text that will classify based on Sentiment Analysis. My question is, can I use text Pre-processing techniques in the sentiment...

11 January 2021 5,932 7 View

Will increasing the number of synonyms in training set increase classifier efficiency?

Hello, If I increased the number of keywords (Features) of examples in training set pertaining to a particular class regarding to Sentiment Analysis Classification , will increase the efficiency...

11 January 2021 5,299 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Venkatesh Sankarasetty

i am using Trypan blue in media for observing colony morphology of oral bacteria in agar plate(0.075 gms/lt). they are forming blue colonies.you can try that.

Massimo Trotta

Dear Hamed,

we have successfully used fluorescein up to 100 micromolar concentration with no harm to alfa-proteobacteria (Rb. sphaeroides)