Is anyone aware of any freely available databases of normed works of art?

I am specifically interested in works of art for which ratings on multiple dimensions are readily available--for instance, ratings of beauty or of liking that were acquired from a sample of...

18 January 2021 296 5 View

Protein Quantification Outside Standard Curve?

I am running a Bradford protein assay and my protein values are falling beyond the standard curve. I am already using 1uL of sample in the standard microplate assay. Is there a way to extend the...

13 December 2020 6,672 3 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Cs-Gluconate for intracellular solution?

Dear patch-clamp enthusiasts, For patch-clamp recordings of vomeronasal sensory neurons, I need a cesium-based intracellular solution. Because I also need a defined amount of chloride, I have to...

11 November 2020 6,384 7 View

PLS- Is it normal that the standardized regression coefficients of my model do not coincide with the VIP values?

I am trying to perform a partial least square model (PLS) using one response variable and 33 explanatory.. Before the analysis I have standardized all my data so i am expecting that the regression...

19 October 2020 6,702 2 View

Which is preferable for whole rat brain clearing: tert-butanol or xylene?

I am performing LM on whole rat brains with a specific focus on the hippocampus and cannot find which information on which clearing technique is better.

05 October 2020 1,306 1 View

Is recoding negative questions always necessary when downloading to SPSS from SurveyMonkey?

I formatted a survey in SurveyMonkey using questions from a valid and reliable instrument (Principles of Adult Learning, Conti, 1978). Questions used a 6 point Likert scale (Always, Almost...

29 September 2020 8,194 4 View

Clarification on Shapiro-wilk sig. , skewness and kurtosis?

Hello, Maybe is a bit silly question but, I have a variable (N=78) and on shapiro-wilk test the sig. is .008 which is just over (greater) than 0.05 meaning that is just about normally...

29 August 2020 1,384 4 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Google Earth Engine for Multitemporal analysis (Land Use Land Cover Change)?

Hello. I am reading about Google Earth Engine (GEE) and their applications for vegetation, landcover and agriculture mapping. I am analyzing an area of 20Mha to determine a multitemporal changes,...

01 May 2020 2,059 11 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View