I just want a quick reference I can search in or a website to know the best PCR technique to detect certain bacterial types or species in a sample, I mean as a detection technique.
This depends on the bacteria you are looking for. The most practical is to amplify the 16S gene by PCR endpoint, send the amplified to sequencing and enter the sequence in Blastn.
Or you can look for papers that mention the primers of a gene that only has the bacteria you are looking for. For example, the Staphylococcus aureus spa gene.
I hope I have been helpful.
Hi. If you want to learn about PCR of 16s regions, PCR primers and bacteria identification I recommend you this paper with more than 1700 citations...a must to read:
https://www.sciencedirect.com/science/article/pii/S0167701203002276
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