Do you use a more convenient and more straightforward method to separate

leukocytes in flow cytometric determination of anti-leukocyte antibodies?

In our practice, we use phycol-verographine separation solution (ρ

=1,088g/cm3).

What we see as a problem is that the method requires four blood samples: 3 for

direct immunofluorescence (for separation) and 1 native for indirect IF,

which is difficult to obtain, especially from young children.