Is there an alternate protocol to CsCl density gradient centrifugation for isolation of Bacteriophages from stool samples?

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Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

Patriotisme ou Nationalisme économique ?

Patriotisme et Nationalisme économique Le patriotisme économique promeut une politique industrielle qui sort du modèle classique et redéfinie les relations socio-économiques entre les nations,...

20 June 2024 6,118 1 View

How to simulate a metalens with backward propagating source using a Lumerical FDTD?

Dear Sir/Madam, I am currently working on a beam shaping problem and have referenced the Metalens example provided on the Ansys Lumerical website. In that example, a forward propagating plane wave...

14 June 2024 4,333 0 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

Which MWCO filter to use to get rid of unwanted proteins?

I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...

05 June 2024 691 1 View

How do you add a new Institution to ResearchGate?

How do new institutions become listed on ResearchGate, please? Where can you add the logo and details for other researchers to find your university or college?

30 May 2024 5,836 2 View

Est-il possible de penser que le capitalisme est en déclin avec la motée du nationalisme économique?

De plus en plus il est observé dans divers coins du monde un retour au nationalisme économique. Des revendications multiples fusent des politiques et même des populations sur les habitudes de...

29 May 2024 5,156 4 View

Salut, La conférence pourrait-elle développer les questions de culture financière ?

Pour l'Afrique centrale d’où je suis les populations restent en marge des activités économiques formelles qui impulserait le développement du fait du manque de culture financière. Un marché...

29 May 2024 5,154 0 View

I need EEG data set for seizures, sleep, awake and epilepsy. In which open source i can access the data?

Electroencephalography (EEG) data set for awake, sleep, and seizures

23 May 2024 7,148 0 View

How can we integrate indigenous knowledge with scientific methods for sustainable local food systems?

How can farmers integrate indigenous knowledge with scientific methods for sustainable local food systems?

19 May 2024 6,038 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

How can I calculate formation energy using VASP-Ab-initio?

I would like to calculate the formation energy of P2-Na0.67Fe0.5Mn0.5O2 based on DFT, what should I do step by step. Any help would be appreciated. Thanks.

29 July 2024 8,248 2 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View