I think it can be analyzed quantitatively by TLC scanner (densitometry method) after sprayed using Dragendorffs
I think HPTLC is method of choice. If you want to try by spectrophotometer then try without adding dragendroffs.
I agree with Prof Sasongko, but the challenge is going to be the extraction of lupinine from your medium, the sensitivity of the method and possible false positives (by interference with non-quinolizidine, draagendorff-reactive compounds) in the medium. This paper may be useful, although not directly applicable to your situation:
Ganzera M1, Krüger A, Wink M. Determination of quinolizidine alkaloids in different Lupinus species by NACE using UV and MS detection. J Pharm Biomed Anal. 2010 Dec 15;53(5):1231-5. doi: 10.1016/j.jpba.2010.05.030. Epub 2010 Jun 8.
Dragendorff Reagent impart colour after reacting with alkaloids, but it impart colour after reacting to other phyto-chemicals too. Spectrophotometer can not separate to all of them hence there is no way to estimate lupinine using spectrophotomete in cell culture.
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